Expression patterns of white blood cells in response to individual food treatments relative to LPS treatment and untreated in an FPIES subject.. Expression patterns of white blood cells in response to individual food treatments relative to LPS treatment and untreated in an FPIES subject.

Total blood white blood cells from a FPIES subject were treated with individual treatmens of 2 foods that were safe for the subject (pear and breast milk), two foods that were triggers (quinoa and sweet potato), and 19 foods of unknown status for the subject. These treatments were compared to LPS treatment and untreated. Overall design: Blood was drawn from FPIES subject who was on trigger-food avoidance, the WBCs isolated by ACK buffer RBC lysis, and the WBCs plated in 12-well plate wells, and treated with individual food treatments, LPS or untreated.

本数据集针对食物蛋白诱导的肠结肠炎综合征（Food Protein-Induced Enterocolitis Syndrome，FPIES）患者的外周血白细胞开展体外处理实验：分别采用2种对该患者安全的食物（梨、母乳）、2种致敏触发食物（藜麦、甘薯）以及19种致敏性未知的食物进行单独处理，并设置脂多糖（Lipopolysaccharide，LPS）处理组与未处理组作为对照。实验整体设计：从严格规避致敏触发食物的FPIES患者体内采集外周血，经ACK缓冲液红细胞裂解法分离白细胞，将白细胞接种于12孔板（12-well plate），随后分别施加单一食物处理剂、脂多糖处理或不做任何处理。