Saccharomyces cerevisiae BY4741 Raw sequence reads. Saccharomyces cerevisiae BY4741

We have isolated Hypoculoside, a new glycosidic amino alcohol lipid from the fungus Acremonium sp. F2434 belonging to the order Hypocreales and determined its structure by 2D-NMR (Nuclear Magnetic Resonance) spectroscopy. Hypoculoside has antifungal, antibacterial and cytotoxic activities. Homozygous profiling (HOP) of hypoculoside in Saccharomyces cerevisiae (budding yeast) revealed that several mutants defective in vesicular trafficking and vacuolar protein transport are sensitive to hypoculoside. Staining of budding yeast cells with the styryl dye FM4-64 indicated that hypoculoside damaged the vacuolar structure. Furthermore, the propidium iodide (PI) uptake assay showed that hypoculoside disrupted the plasma membrane integrity of budding yeast cells. Interestingly, the glycosidic moiety of hypoculoside is required for its deleterious effect on growth, vacuoles and plasma membrane of budding yeast cells.

本研究从隶属于肉座菌目（Hypocreales）的支顶孢属（Acremonium）菌株F2434中分离得到一种新型糖苷类氨基醇脂质类化合物Hypoculoside，并通过二维核磁共振（2D-NMR，Nuclear Magnetic Resonance）光谱技术解析了其化学结构。该化合物Hypoculoside具有抗真菌、抗菌及细胞毒活性。通过对酿酒酵母（Saccharomyces cerevisiae，又称出芽酵母）开展纯合缺失谱分析（Homozygous Profiling, HOP），发现多个囊泡运输及液泡蛋白转运缺陷的突变株对Hypoculoside敏感。采用苯乙烯类染料FM4-64对出芽酵母细胞进行染色实验，结果显示Hypoculoside会破坏酵母的液泡结构。此外，碘化丙啶（PI）摄取实验表明，Hypoculoside会破坏出芽酵母细胞的质膜完整性。值得注意的是，Hypoculoside的糖苷基团是其对出芽酵母的生长、液泡结构及质膜产生有害作用的必要结构基础。