Transcriptional changes in the primary somatosensory cortex upon sensory deprivation. Mus musculus

Experience-dependent plasticity (EDP) is essential for anatomical and functional maturation of sensory circuits during development and can be readily studied is the rodent barrel cortex. Using this model we aimed to uncover changes on the transcriptome level and applied RNA sequencing upon altered sensory experience in juvenile mice in a cortical column and layer specific manner. From column- and layer-specific barrel cortical tissue, high quality RNA was purified and sequenced. The current dataset entails an average of 50 million paired-end reads per sample, 75 base pairs in length. Overall design: Wild type mice were deprived of their C-row whiskers from P12 until P23-P24, after which acute brain slices were prepared and tissues were excised from L2/3 and L4 from specific barrel columns. RNA isolated from these tissue sections was then subjected to RNA-sequencing.

经验依赖性可塑性（Experience-dependent plasticity, EDP）对于发育过程中感觉环路的解剖学与功能成熟至关重要，且可通过啮齿类动物桶状皮层（rodent barrel cortex）便捷开展研究。本研究依托该模型，旨在揭示转录组水平的变化，并以皮层柱及层特异性的方式，对感觉经验改变后的幼年小鼠皮层组织进行RNA测序（RNA sequencing）。研究人员从柱特异性与层特异性的桶状皮层组织中纯化得到高质量RNA并完成测序。本数据集的每个样本平均包含5000万条双端测序读段（paired-end reads），读段长度为75个碱基对（base pairs）。实验整体设计如下：野生型小鼠自P12至P23-P24期间被剥夺C行胡须（C-row whiskers），随后制备急性脑切片，并从特定桶状柱的L2/3层与L4层中提取组织。将从这些组织切片中分离得到的RNA进行RNA测序。