IL-18 nAbs block deleterious inflammatory cytokines induced by acute β-AR stimulation in heart. Mus musculus

To directly test whether inhibition of IL-18 by IL-18 neutralizing monoclonal antibodies (IL-18 nAbs) is sufficient for blocking the inflammatory responses of heart caused by acute β-adrenergic insult, mice were subjected to intraperitoneal injection with rat IL-18 nAbs or IgG as a negative control 1hr after isoproterenol treatment. Overall design: 16 samples. There are 4 groups: vehicle group, mice were subcutaneous injected with a single dose of saline (10 ml/kg body weight), ISO group, mice were subcutaneous injected with a single dose of isoproterenol (5 mg/kg body weight). ISO+IL-18 nAbs group, IL-18 nAbs were intraperitoneally injected (50 μg/ mouse) at 1 hr after ISO injection. ISO+IgG group, rat IgG were intraperitoneally injected (50 μg/ mouse) at 1 hr after ISO injection. The hearts from mice were harvested at 24 hrs after saline or ISO treatment.

为直接验证通过IL-18中和性单克隆抗体（IL-18 nAbs）抑制IL-18是否足以阻断急性β-肾上腺素能刺激引发的心脏炎症反应，研究人员在异丙肾上腺素处理1小时后，对小鼠施以大鼠IL-18 nAbs或IgG阴性对照的腹腔注射。
实验整体设计：共包含16个样本，分为4组：
1. 溶剂对照组（vehicle group）：小鼠皮下单次注射生理盐水（10 ml/kg体重）；
2. 异丙肾上腺素组（ISO组）：小鼠皮下单次注射异丙肾上腺素（5 mg/kg体重）；
3. ISO+IL-18 nAbs组：于ISO注射后1小时，对小鼠腹腔注射IL-18 nAbs（50 μg/只）；
4. ISO+IgG组：于ISO注射后1小时，对小鼠腹腔注射大鼠IgG（50 μg/只）。
在生理盐水或ISO处理后24小时，采集小鼠心脏组织。