Breast tumor miRNA profiling. Homo sapiens

Comparison of miRNA expression profiles in breast tumors clinically annotated for ErbB2, ER, and PR expression, as well as additional parameters. Keywords: Expression profiling of breast tumor biopsies Overall design: MicroRNA was extracted from 500 ng of Trizol extracted total RNA from fresh frozen sections of breast tumor biopsies using the mirVana miRNA Isolation kit from Ambion. 10 ng of enriched miRNA was amplified using the SenseAmp Plus protocol for miRNA from Genisphere Inc. 500 ng of amplified miRNA was labeled using the Array900 miRNA RT kit from Genisphere and hybridized to custom miRNA microarrays.

针对经临床注释标注了表皮生长因子受体2（ErbB2）、雌激素受体（ER）、孕激素受体（PR）表达情况及其他相关参数的乳腺肿瘤样本，对比其微小RNA（miRNA）表达谱。
关键词：乳腺肿瘤活检组织表达谱分析
实验设计方案：从乳腺肿瘤活检组织新鲜冰冻切片中提取总RNA，取500纳克经Trizol法提取的总RNA，使用安进（Ambion）公司的mirVana miRNA分离试剂盒分离微小RNA；取10纳克富集得到的微小RNA，采用Genisphere公司的SenseAmp Plus微小RNA扩增方案进行扩增；取500纳克扩增后的微小RNA，使用Genisphere公司的Array900 miRNA逆转录试剂盒完成标记，随后与定制化微小RNA微阵列芯片进行杂交。