Bulk ATAC-Seq on sorted cord blood hematopoietic populations
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https://www.omicsdi.org/dataset/ega/EGAS00001004742
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Bulk ATAC-Seq was performed on human cord-blood derived hematopoietis popuations using the following markers:
LT-HSC; CD34+CD38-CD45RA-CD90+CD49f+
ST-HSC; CD34+CD38-CD45RA-CD90-CD49f-
MLP; CD34+CD38-CD45RA+
CMP; CD34+CD38+ CD10-CD45RA-Flt3+CD71-
GMP; CD34+CD38+ CD10-CD45RA+ CD71-
MEP; CD34+CD38+ CD10-CD45RA-Flt3-CD71+
T cell; CD3+CD19-
B cell; CD19+CD3-
NK cell; CD56+CD3-CD19-CD14-
Granulocyte; CD14+CD15+CD3-CD19-
Monocyte; CD14+CD15-CD3-CD19-
DC; CD11c+CD45+
Erythroid cell; CD71+GPA+EGA study EGAS00001004742
本研究采用批量转座酶可及性染色质测序(Bulk ATAC-Seq)技术,对人脐带血来源的造血细胞群进行检测,所用细胞分选标记如下:
长期造血干细胞(LT-HSC):CD34+CD38-CD45RA-CD90+CD49f+
短期造血干细胞(ST-HSC):CD34+CD38-CD45RA-CD90-CD49f-
多淋巴定向祖细胞(MLP):CD34+CD38-CD45RA+
共同髓系祖细胞(CMP):CD34+CD38+ CD10-CD45RA-Flt3+CD71-
粒细胞-巨噬细胞祖细胞(GMP):CD34+CD38+ CD10-CD45RA+ CD71-
巨核红细胞祖细胞(MEP):CD34+CD38+ CD10-CD45RA-Flt3-CD71+
T细胞:CD3+CD19-
B细胞:CD19+CD3-
自然杀伤细胞(NK细胞):CD56+CD3-CD19-CD14-
粒细胞:CD14+CD15+CD3-CD19-
单核细胞:CD14+CD15-CD3-CD19-
树突状细胞(DC):CD11c+CD45+
红系细胞:CD71+GPA+;本研究数据已存档于欧洲基因组-表型组档案馆(EGA),存档编号为EGAS00001004742
创建时间:
2020-10-22



