Bulk ATAC-Seq on sorted cord blood hematopoietic populations
收藏资源简介:
Bulk ATAC-Seq was performed on human cord-blood derived hematopoietis popuations using the following markers: LT-HSC; CD34+CD38-CD45RA-CD90+CD49f+ ST-HSC; CD34+CD38-CD45RA-CD90-CD49f- MLP; CD34+CD38-CD45RA+ CMP; CD34+CD38+ CD10-CD45RA-Flt3+CD71- GMP; CD34+CD38+ CD10-CD45RA+ CD71- MEP; CD34+CD38+ CD10-CD45RA-Flt3-CD71+ T cell; CD3+CD19- B cell; CD19+CD3- NK cell; CD56+CD3-CD19-CD14- Granulocyte; CD14+CD15+CD3-CD19- Monocyte; CD14+CD15-CD3-CD19- DC; CD11c+CD45+ Erythroid cell; CD71+GPA+EGA study EGAS00001004742
本研究采用批量转座酶可及性染色质测序(Bulk ATAC-Seq)技术,对人脐带血来源的造血细胞群进行检测,所用细胞分选标记如下: 长期造血干细胞(LT-HSC):CD34+CD38-CD45RA-CD90+CD49f+ 短期造血干细胞(ST-HSC):CD34+CD38-CD45RA-CD90-CD49f- 多淋巴定向祖细胞(MLP):CD34+CD38-CD45RA+ 共同髓系祖细胞(CMP):CD34+CD38+ CD10-CD45RA-Flt3+CD71- 粒细胞-巨噬细胞祖细胞(GMP):CD34+CD38+ CD10-CD45RA+ CD71- 巨核红细胞祖细胞(MEP):CD34+CD38+ CD10-CD45RA-Flt3-CD71+ T细胞:CD3+CD19- B细胞:CD19+CD3- 自然杀伤细胞(NK细胞):CD56+CD3-CD19-CD14- 粒细胞:CD14+CD15+CD3-CD19- 单核细胞:CD14+CD15-CD3-CD19- 树突状细胞(DC):CD11c+CD45+ 红系细胞:CD71+GPA+;本研究数据已存档于欧洲基因组-表型组档案馆(EGA),存档编号为EGAS00001004742



