Development of tools for cell-type specific labeling of neocortical neurons

A complete epigenomic map of human brain cell types will be necessary to fully understand their genetic regulation, and to create the next generation of non-species-restricted genetic tools for their functional characterization. Here we performed both bulk and single-nucleus ATAC-seq studies of fresh human neocortical samples excised during neurosurgery, in order to discover the functional genomic elements that discriminate cell types. This dataset contains 78 bulk layer-dissected ATAC-seq samples, and 3660 single-nucleus ATAC-seq samples from fresh acutely isolated human neurosurgical samples. Furthermore, from these data we identified and validated several enhancers for use in AAV context. We also provide validation single cell RNA-seq for 456 human acutely labeled neurosurgical... (for more see dbGaP study page.)

要全面阐明人类脑细胞类型的基因调控机制，并开发用于其功能表征的下一代无物种限制性遗传工具，完整的人类脑细胞类型表观基因组图谱必不可少。本研究对神经外科手术中切除的新鲜人类新皮质样本同时开展了批量ATAC测序（bulk ATAC-seq）与单细胞核ATAC测序（single-nucleus ATAC-seq）分析，以期筛选可区分细胞类型的功能基因组元件。本数据集包含78份经皮层分层解剖的批量ATAC-seq样本，以及3660份来自新鲜急性分离的人类神经外科样本的单细胞核ATAC-seq样本。此外，本研究从上述数据中筛选并验证了数个可应用于腺相关病毒（adeno-associated virus, AAV）载体系统的增强子。本研究还提供了456份经急性标记的人类神经外科样本的验证用单细胞RNA测序（single-cell RNA-seq）数据……（更多详情请参见dbGaP研究页面）