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Additional file 10: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing

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DataCite Commons2024-12-16 更新2024-07-25 收录
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Accuracy outliers. Genes were identified as accuracy outliers if its median fold deviation, taken across dilution replicates, was contained in the upper or lower 1%ile of all considered genes (see Methods ). Columns labeled by single-cell protocol contain an “H” if a gene was identified as an overestimated outlier, and an “L” if a gene was identified as an underestimated outlier. “Gene set” indicates whether gene is classified as computationally unambiguous (1) or not (2). (XLS 218 kb)

准确率异常值(Accuracy outliers):若某基因在所有稀释重复样本中的中位倍数偏差处于全部待测基因的上下1%分位数区间内,则该基因被判定为准确率异常值(详见方法部分)。以单细胞实验方案(single-cell protocol)命名的列中,若基因被判定为高估型异常值,则标注为"H";若为低估型异常值,则标注为"L"。"基因集(Gene set)"字段用于标注某基因是否为计算上无歧义的基因:若是则赋值为1,否则赋值为2。(XLS 218 KB)
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Figshare
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2017-12-19
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