Database on the amylolytic activity of malted triticale (Bicentenario variety) germinated for 5–8 days, aqueous malt extracts, and immobilization on a cross-linked gelatin support

The database comprises a detailed experimental set on the amylolytic activity of malted triticale (Bicentenario variety) germinated for 5 to 8 days, including physicochemical and enzymatic variables. It reports the protein and moisture contents of seeds and malts, as well as the diastatic power as an indicator of overall enzymatic activity. From aqueous extracts obtained under different time and temperature conditions, total solids and reducing sugar contents were determined. In addition, the activities of α-amylase, β-amylase, and amyloglucosidase were analyzed in both crude extracts and those purified using aqueous two-phase systems (ATPS) composed of ethanol and trisodium citrate, including kinetic parameters (Vmax and Km). Protein concentration and pH were also measured in these systems. Finally, a cross-linked gelatin type B support, stabilized with CaCl₂, was characterized for the immobilization of enzymes derived from the day-7 extract (30 min/40 °C), determining solubility, water absorption, immobilized enzymatic activity, immobilization yield, residual activity over five reuse cycles, and the effect of enzymatic supplementation on the release of reducing sugars in barley wort.

本数据集收录了一套针对萌发5至8天的百周年（Bicentenario）品种小黑麦麦芽淀粉水解活性的详细实验数据，涵盖理化与酶学相关参数。该数据集记录了原种子与麦芽的蛋白质及水分含量，同时记录了作为整体酶活性指标的糖化力（diastatic power）。针对不同时间与温度条件下获得的水提物，测定了其总固体含量与还原糖含量。此外，本研究针对粗提物，以及采用乙醇与柠檬酸三钠（trisodium citrate）构建的水两相系统（aqueous two-phase systems, ATPS）纯化得到的提取物，均开展了α-淀粉酶（α-amylase）、β-淀粉酶（β-amylase）与淀粉葡萄糖苷酶（amyloglucosidase）的活性分析，并同步测定了相关动力学参数（Vmax与Km）。同时测定了上述系统中的蛋白质浓度与pH值。最后，本研究对以氯化钙（CaCl₂）稳定的交联B型明胶载体开展了表征，该载体用于固定化取自第7天提取物（30 min/40 °C）的酶类；本研究同时测定了载体的溶解度、吸水率、固定化酶活性、固定化产率、五次重复使用循环后的残留活性，以及酶补加对大麦麦芽汁（barley wort）中还原糖释放的影响。