Ambiguous sequences at different cut off levels - (Item J)

For the evaluation of ambiguous sequences in microbial reference genomes we assessed different cut of levels for the generation of a curated microbial genome database. For each sequence fragment we determined the ratio between the number of negative BLAST hits (query genus ≠ subject genus) and all BLAST hits.<br><br>For a detailed description of this analysis, see the Supplemental Materials section of the publication.<br><br><b>Reference</b> <b>Kirstahler P, Bjerrum SS, Friis-Møller A, </b><b>la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2017) </b>Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid.<b> Journal </b>Vol:Issue page-page.

为评估微生物参考基因组中的模糊序列，我们针对构建经过人工整理的微生物基因组数据库的不同截断阈值开展了评估。针对每一段序列片段，我们计算了阴性BLAST（Basic Local Alignment Search Tool）比对结果（查询序列所属属与比对靶序列所属属不一致）的数量与总BLAST比对结果数量之间的比值。

有关本次分析的详细说明，请参阅该文献的补充材料部分。

<b>参考文献</b> <b>Kirstahler P, Bjerrum SS, Friis-Møller A, </b><b>la Cour M, Aarestrup FM, Westh H. 及 Pamp SJ. (2017)</b> 《基于基因组学的人眼体液微生物鉴定》。<b>期刊</b> 卷:期 页码-页码。