MinION 1D² Reads From Mus musculus GL261 Cell Lines

Raw FAST5 MinION cDNA reads (1D²) from a murine GL261 neuroblastoma cell line, cultured at the Malaghan Institute of Medical Research, sequenced on a R9.5 flow cell in August 2017 using the LSK309 1D² kit for ligating ONT adapters to cDNA generated using strand-switching primers.

This dataset currently only includes a subset of the total reads: reads from one run that mapped (in whole or in part) to a mouse beta-actin transcript [ENSMUST00000100497.10].

本数据集包含源自小鼠GL261神经母细胞瘤细胞系的原始FAST5格式MinION测序互补DNA（cDNA）1D²读段。该细胞系于马拉汉医学研究所（Malaghan Institute of Medical Research）完成培养，于2017年8月使用LSK309 1D²试剂盒将牛津纳米孔（ONT）接头连接至经链转换引物合成的cDNA，并在R9.5型流动槽上完成测序。
当前本数据集仅包含总读段的一个子集：即单次测序运行中，全部或部分比对至小鼠β-肌动蛋白转录本[ENSMUST00000100497.10]的读段。