EURGen-RefLabCap External Quality Assessment (EQA) 2023

The EURGen-RefLabCap project is complementary to the European Centre of Disease Prevention and Control (ECDC) European Antimicrobial Resistance Genes Surveillance Network (EURGen-Net). The project aims at improving capacities of National Reference Laboratories (NRLs) in European countries for identification and for phenotypic and genotypic characterization of carbapenem-resistant Enterobacterales (CRE) and colistin-resistant CRE (CCRE), as well as carbapenem- and/or colistin-resistant Pseudomonas aeruginosa (C/CRPa) and Acinetobacter baumannii complex (C/CRAb). Furthermore, the project aims at strengthening capacities for national surveillance and outbreak investigation of CRE/CCRE, C/CRPa and C/CRAb, and improve the availability and quality of European-level molecular surveillance data. One of the main goals of the EURGen-RefLabCap project is to support modernisation of?diagnostic and molecular typing tests using whole-genome sequencing (WGS) analytical methods to achieve those respective aims. External quality assessment (EQA) exercises are an important tool to assess the capacity of laboratories to follow their own routine procedures and obtain accurate results. This assessment is done by comparing the achieved results with expected results produced by standard methods, and with results obtained by other laboratories. EQAs may also allow for comparing the performance and accuracy of different laboratory protocols and pipelines for analysis of WGS data. This can be possible if the results submitted by participants, for the same type of analyses, are obtained using different methods.

EURGen-RefLabCap项目（EURGen-RefLabCap）与欧洲疾病预防控制中心（European Centre of Disease Prevention and Control, ECDC）主导的欧洲抗菌药物耐药基因监测网络（European Antimicrobial Resistance Genes Surveillance Network, EURGen-Net）形成互补。本项目旨在提升欧洲各国国家参考实验室（National Reference Laboratories, NRLs）对碳青霉烯类耐药肠杆菌科（carbapenem-resistant Enterobacterales, CRE）、黏菌素耐药CRE（colistin-resistant CRE, CCRE），以及碳青霉烯类和/或黏菌素耐药铜绿假单胞菌（carbapenem- and/or colistin-resistant Pseudomonas aeruginosa, C/CRPa）和鲍曼不动杆菌复合体（Acinetobacter baumannii complex, C/CRAb）的鉴定、表型与基因型表征能力。此外，本项目还旨在强化针对CRE/CCRE、C/CRPa及C/CRAb的国家监测与暴发调查能力，并提升欧洲层面分子监测数据的可用性与质量。EURGen-RefLabCap项目的核心目标之一，是依托全基因组测序（WGS）分析方法推动诊断与分子分型检测技术的现代化升级，以达成上述各项目标。室间质评（External Quality Assessment, EQA）是评估实验室遵循常规操作流程并获取准确检测结果能力的重要工具，此类评估通过将参评实验室所得结果与标准方法生成的预期结果、以及其他实验室的检测结果进行比对来完成。若参与者针对同类分析采用了不同的检测方法，室间质评还可用于对比不同实验室分析流程及全基因组测序数据分析管线的性能与准确性。