Data Sheet 1_Identification and expression responses of TCP gene family in Opisthopappus taihangensis under abiotic stress.pdf

The TCP gene family plays pivotal roles in the development and abiotic stress responses of plants; however, no data has been provided for this gene family in Opisthopappus taihangensis. Based on O. taihangensis genome, 14 TCP genes were identified and divided into two classes (I and II). After tandem and segmental duplication/whole-genome duplication (WGD), more loss and less gain events of OtTCPs occurred, which might be related with the underwent purifying selection during the evolution. The conserved motifs and structures of OtTCP genes contained light response, growth and development, hormone response, and stress-related cis-acting elements. Different OtTCP genes, even duplicated gene pairs, could be expressed in different tissues, which implied that OtTCP genes had diverse function. Among OtTCPs, OtTCP4, 9 and 11 of CYC clade (Class II) presented a relative wide expression pattern with no or one intron. The three TCP genes could be regarded as important candidate factors for O. taihangensis in growth, development and stress response. These results provided some clues and references for the further in-depth exploration of O. taihangensis resistance mechanisms, as well as those of other unique eco-environment plants.

TCP基因家族（TCP gene family）在植物生长发育与非生物胁迫响应中发挥关键作用；然而目前尚无针对太行菊（Opisthopappus taihangensis）该基因家族的相关研究数据。基于太行菊基因组，本研究共鉴定出14个TCP基因，并将其划分为两类（I类与II类）。经串联重复、片段重复及全基因组复制（whole-genome duplication, WGD）事件分析后发现，太行菊TCP基因（OtTCPs）的丢失事件多于获得事件，这可能与其进化过程中经历的纯化选择作用有关。OtTCP基因的保守基序与基因结构包含光响应、生长发育、激素响应及胁迫相关的顺式作用元件。不同的OtTCP基因，甚至包括重复基因对，均可在不同组织中表达，这表明OtTCP基因的功能具有多样性。在OtTCP家族中，属于II类CYC进化枝的OtTCP4、OtTCP9与OtTCP11基因不含内含子或仅含1个内含子，且表达模式相对广泛。上述3个TCP基因可作为太行菊生长、发育及胁迫响应过程中的重要候选因子。本研究结果为深入解析太行菊及其他独特生态环境植物的抗性机制提供了线索与参考依据。