Zebrafish embryo T18°C MAX QUANT

Effect of temperature variation on zebra fish health and behaviour Water temperature is an important environmental parameter that influences the distribution and the health of fishes. Moreover, temperature has a central role in ectothermic animals affecting their physiology and behaviour. The aim of this study was to determine the effects of ambient temperature on the molecular mechanism and the behavioural responses in ZF (Danio rerio) (ZF) a widely used animal model for environmental "omics" genomics researches. A global proteomic analysis was performed on fish brains to investigate physiological and biochemical changes that may occur from the exposure to this environmental stressor at the central nervous system level. Behavioural investigations were performed using a Y-Maze task to evaluate how temperature variations may affect swimming performance, the response to novelty and the spatial memory. Adult specimens of wild type ZF were kept at three different temperatures: 18 °C, 34 °C and 26 °C (used as a control) for 21 days. Proteomic data revealed 202XXX differentially expressed proteins across acclimation groups. These proteins are involved in energy metabolism, mitochondrial regulation and cytoskeletal organization. Temperature variation was able to alter swimming performance in adult ZF and to induce variations in the novel ambient exploration and in the acquisition and consolidation of spatial memory. This study indicates that moderate temperature variations can elicit biochemical changes that may affect fish health and behaviour. This combined approach may provide insights into mechanisms supporting thermal adaptation and plasticity in fishes.

水温变化对斑马鱼健康与行为的影响。水温是影响鱼类分布与健康状态的重要环境参数。此外，温度对于变温动物的生理机能与行为表现具有核心调控作用。本研究旨在探究环境温度对斑马鱼（ZF，Danio rerio）分子机制与行为响应的影响——斑马鱼是环境组学（omics）与基因组学研究中广泛应用的模式动物。研究团队通过对斑马鱼脑组织开展全局蛋白质组学分析，探究中枢神经系统在该环境胁迫下可能发生的生理与生化变化。采用Y迷宫任务开展行为学实验，以评估水温变化如何影响斑马鱼的游泳能力、新异环境探索行为与空间记忆能力。将野生型成年斑马鱼分别置于18℃、34℃与作为对照组的26℃环境中驯养21天。蛋白质组学数据显示，不同驯养温度组间共存在202XXX个差异表达蛋白。这些差异蛋白参与能量代谢、线粒体调控与细胞骨架组织等生物学过程。水温变化可改变成年斑马鱼的游泳能力，并引发新环境探索行为、空间记忆的获取与巩固过程出现异常变化。本研究表明，适度的水温变化可诱发影响鱼类健康与行为的生化改变。这种结合组学与行为学的研究策略，可为解析鱼类的温度适应与可塑性机制提供全新的研究视角。