RNA-sequencing of EpH4 and EpRas cells treated with Etv4 and Etv5 siRNAs and/or TGF-beta.. Mus musculus

We performed RNA-seq analysis in mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) to analyze the mechanisms of transcriptional regulation during TGF-beta-induced EMT. Overall design: RNA-seq was perfomed using EpH4 and EpRas cells transfected with Etv4 and Etv5 siRNAs and stimulated with 1 ng/ml TGF-beta or left untreated for 48 h.本研究以小鼠乳腺上皮EpH4细胞系及其经Ras转化的衍生细胞株（EpRas）为研究对象，开展RNA测序（RNA-seq）分析，旨在解析转化生长因子β（transforming growth factor-β, TGF-β）诱导的上皮间质转化（Epithelial-Mesenchymal Transition, EMT）过程中的转录调控机制。整体实验设计如下：对EpH4及EpRas细胞转染Etv4与Etv5小干扰RNA（small interfering RNA, siRNA）后，分别用1 ng/ml的TGF-β刺激处理，或不施加任何处理，持续培养48小时后进行RNA-seq检测。