Synthesis of hydrazinylthiazole carboxylates: a mechanistic approach for treatment of diabetes and its complications

The inhibition potential of compounds 3a–n was found to be dose-dependent, as shown in Supplementary Table 2.Compounds 3a–n were tested for their in vitro antiglycation activity using aminoguanidine as reference inhibitor (Supplementary Table 3).The free radical scavenging ability of compounds 3a–n was estimated using the DPPH scavenging model. Ascorbic acid was used as positive control, and percentage antioxidant ability and IC50 values are presented in Supplementary Table 4.

研究发现化合物3a–n的抑制活性呈剂量依赖性，相关实验结果详见补充表2。以氨基胍（aminoguanidine）作为参比抑制剂，对化合物3a–n开展了体外抗糖基化活性检测，相关结果见补充表3。采用2,2-二苯基-1-苦肼基自由基（DPPH）清除模型评估了化合物3a–n的自由基清除能力，以抗坏血酸作为阳性对照，其抗氧化活性百分率及半抑制浓度（IC50）值详见补充表4。