Proteomics data of mouse skeletal muscles (hypergravity exposure) [Reanalysis: JPST000736]

This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Applying proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as osteoporosis occurring on the ground by searching serum, bone and skeletal muscle (soleus, extensor digitorum longus) proteomes. The goal of this study is to get better understanding to protect the health of both astronauts and osteoporosis patients.
Proteome analyses of skeletal muscles from 10 male C57BL/6J mouse (1-g gravity and 3-g gravity [n=5, respectively]) were carried out tryptic digestion followed by LC-MS/MS analysis (DDA). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR threshold by searching against the randomized decoy DB. [Original project description]

本项目为利用国际空间站（International Space Station, ISS）日本希望号实验舱（Kibo Japanese Experiment Module）开展的骨质疏松与骨量相关蛋白医学蛋白质组学分析研究。本研究借助蛋白质组学技术，通过分析血清、骨骼及骨骼肌（比目鱼肌、趾长伸肌）的蛋白质组，旨在鉴定参与航天诱导骨质减少以及地面骨质疏松发生过程的相关蛋白。本研究的目标在于深化对宇航员与骨质疏松患者健康保护相关机制的认知。
本研究对10只雄性C57BL/6J小鼠进行骨骼肌蛋白质组学分析：将小鼠分别置于1g重力与3g重力环境下（每组各5只），先对样本进行胰蛋白酶消化处理，随后采用液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）进行数据依赖型采集（Data Dependent Acquisition, DDA）分析。所得结果通过Mascot软件比对UniProt数据库进行检索，并通过比对随机诱饵数据库，以1%的错误发现率（False Discovery Rate, FDR）阈值对鉴定得到的肽段进行过滤筛选。[原始项目描述]