Effects of chlorothalonil on the skin microbiome of the frog Lithobates vibicarius

Using tadpoles of the Green-eyed frog (Lithobates vibicarius) as our model organism and 16S rRNA amplicon sequencing, we examined the influence of the fungicide chlorothalonil on the skin bacterial communities. Our study reveals the effect of the exposure to environmentally relevant concentrations of fungicides on the skin microbiomes of amphibian early life stage, which may in turn impact the stability of host-microbe interactions and microbiome-fitness correlation

本研究以绿眼蛙（Green-eyed frog，Lithobates vibicarius）的蝌蚪作为模式生物（model organism），采用16S rRNA扩增子测序（16S rRNA amplicon sequencing）技术，探究了杀菌剂百菌清（chlorothalonil）对其皮肤细菌群落的影响。本研究揭示了暴露于环境相关浓度的杀菌剂对两栖动物早期生命阶段皮肤微生物组的影响，该效应或可进而影响宿主-微生物互作（host-microbe interactions）的稳定性以及微生物组-适合度相关性（microbiome-fitness correlation）。