MYCN directly regulates TET1 expression to control 5-hmC distribution and enhance neuroblastoma cell migration in hypoxia [ChIP-Seq]. MYCN directly regulates TET1 expression to control 5-hmC distribution and enhance neuroblastoma cell migration in hypoxia [ChIP-Seq]

We performed HIF-1alpha ChIP-seq in SK-N-BE(2) cells cultured in normoxia vs. hypoxia for 48 hrs. We identified two new HIF-1alpha binding sites in TET1 that control TET1expression in hypoxia Overall design: SK-N-BE(2) cells were cultured in normoxia or hypoxia for 48 hours. HIF-1alpha ChIP were performed on fixed chromatin

本研究在常氧（normoxia）与低氧（hypoxia）条件下培养SK-N-BE(2)细胞48小时，针对缺氧诱导因子-1α（HIF-1alpha）开展染色质免疫共沉淀测序（ChIP-seq）实验。
我们在TET1基因中鉴定出两个全新的HIF-1alpha结合位点，这些位点可在低氧条件下调控TET1的表达。
整体实验设计：将SK-N-BE(2)细胞分为常氧、低氧两组，分别培养48小时后，对固定后的染色质进行HIF-1alpha染色质免疫共沉淀实验。