C. elegans small RNA-seq profiling of a null mutant with a deletion of the entire lotr-1 coding sequence

Proteins containing LOTUS and Tudor domains have critical roles in the germline, including in small RNA biology. LOTR-1 is the only protein expressed in the germline of C. elegans which has LOTUS and Tudor domains. Here, to understand the influence of LOTR-1 in small RNA biology, we sequenced small RNAs of wild-type and lotr-1 mutant animals. Small RNA-seq profiling of wild-type and lotr-1 mutant worms in triplicates. The mutant analysed in this experiment has a deletion of the entire lotr-1 coding sequence. RNA was treated with RppH prior to library preparation.

携带LOTUS结构域（LOTUS domain）与Tudor结构域（Tudor domain）的蛋白质在生殖系中具有关键作用，其功能涉及小RNA生物学领域。LOTR-1是秀丽隐杆线虫（C. elegans）生殖系中唯一同时携带LOTUS结构域与Tudor结构域的蛋白质。为明确LOTR-1对小RNA生物学过程的影响，本研究对野生型及lotr-1突变体动物的小RNA进行了测序。本研究对野生型与lotr-1突变型秀丽隐杆线虫开展了三次生物学重复的小RNA测序（small RNA-seq）分析。本实验所用的突变体为完全缺失lotr-1编码序列的突变株。在文库制备前，需使用RppH处理RNA样品。