DMT1 silencing effects in 2D versus 3D cel culture conditions in MDA-MB-231 triple negative breast cancer cells. DMT1 silencing effects in 2D versus 3D cel culture conditions in MDA-MB-231 triple negative breast cancer cells

To investigate the differential transcriptomics upon DMT1 in 2D vs 3D cell culture in breast cancer cells Gene expression analysis from MDA-MB-231 RNA-seq data of WT and DMT1 KO cells both in 2D and 3D cell culture conditions Overall design: Gene expression profiling analysis using data obtained from MDA-MB-231 RNA-seq of 3 replicates of WT and DMT1 KO cells both in 2D and 3D cell culture conditions Raw files from MdaWT and Mda KO (2D cell culture) were truncated and complete raw data are not available.

为探究乳腺癌细胞在2D与3D细胞培养条件下DMT1相关的差异转录组学特征，本研究基于MDA-MB-231细胞的RNA测序（RNA-seq）数据，对2D及3D培养环境下的野生型（Wild Type, WT）与DMT1敲除（Knockout, KO）细胞开展基因表达分析。
实验设计：本研究采用2D及3D细胞培养条件下的野生型与DMT1敲除MDA-MB-231细胞各3次生物学重复的RNA测序数据进行基因表达谱分析。
注：MdaWT与Mda KO（2D细胞培养组）的原始测序文件已被截断，完整原始数据暂不可获取。