miRNA expression profiling in mesenteric arteries of mice infused or not with angiotensin II for 7 and 14 days

We use next-generation sequencing (NGS) to profile miRNA in small arteries of mice infused with angiotensin (Ang) II for 7 and 14 days. The goal is to identify differentially expressed miRNAs that are associated with Ang II-induced vascular injury and hypertension. Overall design: Ten to 12-week-old male C57BL/6J WT (Harlan laboratories, Indianapolis, IN) mice were infused SC with Ang II (1000 ng/kg/min) with ALZET osmotic mini pumps (model 1002, Durect Corporation) for 7 or 14 days. Control mice underwent sham surgery. At the end of the treatments, total RNA was extracted from mesenteric arteries for miRNA profiling using NGS by Illumina HiSeq 2500.

我们采用下一代测序技术（next-generation sequencing, NGS），对经血管紧张素II（angiotensin II, Ang II）持续输注7天及14天的小鼠小动脉中的微小RNA（miRNA）进行谱分析。本研究旨在筛选与血管紧张素II诱导的血管损伤及高血压相关的差异表达微小RNA。

整体实验设计：选取10至12周龄的雄性C57BL/6J野生型（WT）小鼠（购自印第安纳州印第安纳波利斯的Harlan实验室），通过ALZET渗透压微型泵（型号1002，Durect Corporation）以1000 ng/kg/min的速率皮下输注血管紧张素II，持续7天或14天；对照组小鼠接受假手术处理。实验处理结束后，从各组小鼠的肠系膜动脉中提取总RNA，采用Illumina HiSeq 2500平台开展下一代测序，以完成微小RNA谱分析。