Effects of heme oxygenase-1 recombinant Lactococcus lactis on the intestinal barrier of hemorrhagic shock rats

This study aimed to investigate the effects of heme oxygenase-1 recombinant Lactococcus lactis (LL-HO-1) on the intestinal barrier of rats with hemorrhagic shock. One hundred Sprague-Dawley male rats (280–320 g) were randomly divided into healthy control group (N group) and hemorrhagic shock group (H group). Each group was subdivided into HO1t, HO2t, HO3t, PBS and LL groups in which rats were intragastrically injected with LL-HO-1 once, twice and three times, PBS and L. lactis (LL), respectively. The mortality, intestinal myeloperoxidase (MPO) activity, intestinal contents of TNF-α, IL-10 and HO-1, and intestinal Chiu's score were determined. Results showed that in N group, the HO-1 content increased after LL-HO-1 treatment, and significant difference was observed in HO1t group and HO2t group (P<0.05). In H groups, MPO activity and Chiu's score decreased, but IL-10 content increased in LL-HO-1-treated groups when compared with PBS and LL groups (P<0.05). When compared with N group, the MPO activity reduced dramatically in LL-HO-1-treated groups. Thus, in healthy rats (N group), intragastrical LL-HO-1 treatment may increase the intestinal HO-1 expression, but has no influence on the intestinal barrier. In hemorrhagic shock rats, LL-HO-1 may significantly protect the intestinal barrier, and repeating the intragastrical LL-HO-1 treatments twice has the most obvious protection.

本研究旨在探讨重组血红素氧合酶-1乳酸乳球菌（LL-HO-1）对失血性休克大鼠肠屏障的影响。选取100只体重280~320g的斯普拉格-道利（Sprague-Dawley, SD）雄性大鼠，随机分为健康对照组（N组）与失血性休克组（H组）。每组再细分为HO1t、HO2t、HO3t、PBS（磷酸盐缓冲液）组与LL组，各组大鼠分别经胃内单次、两次、三次注射LL-HO-1，以及PBS和乳酸乳球菌（LL）。测定各组大鼠的死亡率、肠髓过氧化物酶（MPO）活性、肠组织肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）及血红素氧合酶-1（HO-1）含量，以及肠组织Chiu评分。结果显示，在N组中，经LL-HO-1干预后HO-1含量升高，其中HO1t组与HO2t组差异具有统计学意义（P<0.05）。在H组中，与PBS组及LL组相比，LL-HO-1干预组的MPO活性与Chiu评分均降低，而IL-10含量升高（P<0.05）。相较于N组，LL-HO-1干预组的MPO活性显著降低。综上，在健康大鼠（N组）中，经胃内给予LL-HO-1可提升肠组织HO-1表达，但对肠屏障无明显影响；在失血性休克大鼠中，LL-HO-1可显著保护肠屏障，且经胃内给予LL-HO-1两次的保护效果最为显著。