Regulation of genes expression in photoreceptor and other retinal cells by histone H3 tri-methylation at K27 during retinal development. Mus musculus

To analyze roles of histone H3 tri-methylation for transcription in retina, global transcription patterns of retinal cells of Ezh2, H3K27 methylase, knockout mice and control were examined Overall design: Examination of global mRNA expression patterns of mouse retinal cells fractioned into Cd73+ photoreceptors and Cd73- other retinal cells at postnatal 12 mouse

为探究组蛋白H3三甲基化（histone H3 tri-methylation）在视网膜转录过程中的功能，本研究对H3K27甲基转移酶（H3K27 methylase）Ezh2基因敲除小鼠及其对照小鼠的视网膜细胞全局转录模式进行了检测。
整体实验设计：对出生后12天的小鼠视网膜细胞进行分选，分为Cd73阳性光感受器细胞与Cd73阴性的其他视网膜细胞，随后检测其全局mRNA表达模式。