Single-cell TCR sequencing of gluten-specific T cells from 20 celiac disease patients uploaded on 2021

To examine the T-cell receptors of the gluten-specific T cells in celiac disease, we isolated single CD4+ T cells from blood , gut biopsies or T-cell lines generated from gut biopsies using HLA-DQ2.5 tetramers and determined the sequences of rearranged TCRalpha and TCRbeta gene pairs.EGA study EGAS00001005047

为探究乳糜泻患者体内麸质特异性T细胞的T细胞受体（T-cell receptor, TCR），我们从血液、肠道活检组织或由肠道活检组织建立的T细胞系中分离单个CD4阳性T细胞，采用HLA-DQ2.5四聚体技术完成分选，并测定重排后的TCRα与TCRβ基因对的序列。本研究对应EGA研究EGAS00001005047。