High-throughput discovery of human antimicrobial peptide activators of Salmonella Typhimurium PhoPQ

We developed a surface-displayed library of 117 human antimicrobial peptides (AMPs) and over 3000 human AMP variants in a laboratory E. coli strain expressing PhoP and PhoQ from Salmonella Typhimurium. We used sort-seq (fluorescence-activated cell sorting followed by next-generation sequencing) to characterize PhoPQ activation by these surface-displayed AMPs. Initial assessment of library diversity, sort-seq characterization

本研究构建了一套表面展示文库，该文库包含117种人源抗菌肽（antimicrobial peptides, AMPs）及超过3000种人源抗菌肽变体，构建工作在一株表达鼠伤寒沙门氏菌PhoP与PhoQ蛋白的实验室大肠杆菌菌株中完成。本研究采用分选测序（sort-seq，即荧光激活细胞分选结合下一代测序）技术，对上述表面展示抗菌肽介导的PhoPQ系统激活效应进行表征。本研究对该文库的多样性开展了初步评估，并完成了分选测序相关表征工作。