Gut microbiome diversity of porcine peritonitis model of sepsis

Animal models are essential in understanding of the mechanisms of sepsis moreover the development and the assessment of emerging therapies. In clinically relevant porcine model, however, a significant variability in the host response has been observed among animals. Thus, there is a strong demand to better understand the potential sources of this heterogeneity. In this study, we compared faecal microbiome composition of 12 animals. Three samples were collected at different time points from each animal. Bacteriome was subjected to 16S rDNA profiling. A significant difference in bacterial composition was associated with the season (p 0.3). The season batch explained 55% of the total variance in the bacteriome diversity. The season term was highly significant from the high-resolution level of the bacterial amplicon sequencing variants up to the level of phylum. The diversity of the microbiome composition could significantly influence experimental model of sepsis, and studies are warranted to demonstrate the effects of gut microbiome diversity on the host-response. If confirmed, control of the gut microbiome should become a standard part of the pre-clinical sepsis experiments.

动物模型是阐明脓毒症（sepsis）发病机制、开发并评估新兴治疗手段的核心研究工具。然而在符合临床相关性的猪模型中，不同个体间的宿主应答存在显著异质性，因此亟需明确该异质性的潜在来源。本研究对12头实验动物的粪便微生物组组成开展了比较分析：每头动物于不同时间点采集三份粪便样本，采用16S核糖体DNA（16S rDNA）测序对细菌组（bacteriome）进行群落谱分析。研究结果显示，细菌群落组成的差异与采样季节显著相关（p=0.3），采样季节批次可解释细菌组多样性总变异的55%。从高分辨率的细菌扩增子测序变异型水平直至门分类学水平，采样季节因素均表现出极高的统计学显著性。粪便微生物组组成的多样性可显著影响脓毒症实验模型，因此亟需开展相关研究以明确肠道微生物组多样性对宿主应答的调控作用。若该结论得以验证，肠道微生物组调控将成为脓毒症临床前实验的标准化流程环节。