RNA-seq analysis of gene expression in adult head of Canton-S and Bully lines

Goal: Identify genes that are differentially expressed in hyper-aggressive Bully line. We used wildtype Canton-S flies as control. We also explored the effect of developmental temperature on gene expression. Total RNA were extracted from 6-day-old adult heads from Canton-S or Bully lines that were raised at 19C or 25C. A total of 4 samples were obtained. For each sample, 2 independent biological replicates were included.

研究目标：鉴定高侵袭性Bully果蝇品系中差异表达的基因。本研究以野生型坎顿S（Canton-S）果蝇作为对照，同时探究发育温度对基因表达的影响。实验材料取自分别在19℃与25℃环境下饲养的坎顿S或Bully品系的6日龄成虫头部，从中提取总RNA，共获得4份样本，每份样本设置2次独立生物学重复。