Gene expression in crossed cerebellar diaschisis in a transient middle cerebral artery occlusion rat model

Crossed cerebellar diaschisis (CCD) is hypoperfusion and hypometabolism in the contralateral cerebellar hemisphere caused by supratentorial lesion. We evaluated chronological change of cerebellar blood flow (CbBF) and gene expressions in cerebellum using a rat model of focal ischemic stroke. Gene expression in rat cerebellar cortex was measured at 2 days after transient right middle cerebral artery occlusion(MCAO). Left (contralateral to the infarct) and right (ipsilateral to the infarct) cerebellar cortex of MCAO group and left cerebellar cortex of control group were extracted. To obtain sufficient mass of the experimental material, tissues from four rats in each group (MCAO or control) were combined into one sample.

交叉性小脑失联络（Crossed cerebellar diaschisis, CCD）指由幕上病变引发的对侧小脑半球低灌注与代谢减低。本研究通过局灶性缺血性脑卒中大鼠模型，评估了小脑血流量（cerebellar blood flow, CbBF）的时序变化及小脑组织的基因表达情况。研究人员在短暂性右侧大脑中动脉闭塞（middle cerebral artery occlusion, MCAO）造模后第2天，检测了大鼠小脑皮层的基因表达水平。分别提取MCAO组大鼠的梗死灶同侧小脑皮层、梗死灶对侧小脑皮层，以及对照组大鼠的左侧小脑皮层。为获取足够的实验组织样本量，将每组（MCAO组或对照组）4只大鼠的组织合并为一个样品。