RNA structure probing in Magnusiomyces capitatus by Oxford nanopore sequencing. RNA structure probing in Magnusiomyces capitatus by Oxford nanopore sequencing

RNA was isolated from yeast Magnusiomycet capitatus. Structurally unconstrained adenosines were modified by diethyl pyrocarbonate (DEPC). Modified sites were identified by comparison of DEPC-treated and control samples using direct RNA sequencing on the Oxford nanopore MinION sequencer, following a protocol by Bizuayehu et al. 2022.

本研究从头状马格努西酵母（Magnusiomycet capitatus）中提取核糖核酸（RNA）。采用焦碳酸二乙酯（diethyl pyrocarbonate, DEPC）对无结构约束的腺苷进行化学修饰。参照Bizuayehu等人2022年发布的实验方案，使用牛津纳米孔MinION测序仪（Oxford nanopore MinION sequencer）开展直接RNA测序，通过对比DEPC处理组与对照组样本，实现修饰位点的鉴定。