Genome-wide maps of H3K9Ac in wild type and sinhcaf mutant zebrafish embryos. Genome-wide maps of H3K9Ac in wild type and sinhcaf mutant zebrafish embryos

H3K9Ac ChIP-Seq was conducted to investigate whether loss of sinhcaf increases the histone acetylation in the promoter region of kinesin family genes.Increased H3K9Ac ChIP-Seq peaks upon sinhcaf loss were detected in the kif26ab promoter region. Overall design: Examination of H3K9Ac in wild type and sinhcaf mutant zebrafish embryos by CHIP-Seq

本研究采用H3K9乙酰化修饰染色质免疫共沉淀测序（ChIP-Seq）技术，旨在探究sinhcaf缺失是否会提升驱动蛋白家族基因启动子区域的组蛋白乙酰化水平。在sinhcaf缺失的样本中，研究人员于kif26ab基因启动子区域检测到H3K9乙酰化修饰染色质免疫共沉淀测序峰信号显著上调。实验整体设计：通过染色质免疫共沉淀测序（ChIP-Seq）分析野生型与sinhcaf突变型斑马鱼胚胎中的H3K9乙酰化修饰水平。