Raw dataset for bacterial exopolysaccharide production using cassava pulp as substrate by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by eight Bacillus spp. isolated from Thai milk kefir [1] on a substrate of cassava pulp. Cassava pulp was analyzed for its moisture, ash, fat, protein, fiber, starch and sugar, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL) when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by several Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and antioxidant activities assessed by DPPH scavenging assay, FRAP assay and hydroxyl radical scavenging activity were collected. Agar disc diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions using the spread plate technique were the primary techniques used.

本原始数据集针对从泰国牛奶开菲尔中分离得到的8株芽孢杆菌属（Bacillus spp.）菌株所产胞外多糖（exopolysaccharide, EPS）的抗氧化与抗菌活性展开分析，其发酵底物为木薯渣。当以木薯渣作为胞外多糖的生产底物时，需对其水分、灰分、脂肪、蛋白质、膳食纤维、淀粉、总糖、中性洗涤纤维（neutral detergent fiber, NDF）、酸性洗涤纤维（acid detergent fiber, ADF）以及酸性洗涤木质素（acid detergent lignin, ADL）进行成分分析。发酵3天后，记录总平板菌落数、还原糖含量、培养液pH值以及胞外多糖的鲜重与干重等相关数据。针对上述多株芽孢杆菌属菌株所产的胞外多糖，本数据集还收集了总酚含量、总黄酮含量、对无乳链球菌（Streptococcus agalactiae）与金黄色葡萄球菌（Staphylococcus aureus）的抗菌活性，以及通过DPPH自由基清除实验、FRAP实验与羟基自由基清除实验评估得到的抗氧化活性数据。本研究采用的主要实验技术包括琼脂纸片扩散法、用于抗氧化活性与生物活性成分检测的分光光度法、常规营养成分分析、3,5-二硝基水杨酸法（dinitrosalicylic acid method）、pH计测定法，以及采用涂布平板法进行梯度稀释后计数总平板菌落数的方法。[1]