CIL:12199, Gallus gallus gallus, memory B cell. In Cell Image Library

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2D panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.

处于有丝分裂期的DT40鸡B淋巴细胞系细胞。本数据集包含采用100×/1.4NA平场复消色差物镜（planapo objective lens）在Deltavision成像系统上采集的宽场免疫荧光图像Z轴序列，并通过约束迭代算法（应用精密公司，Applied Precision）完成反卷积处理。本图展示的为中央Z轴切片。在表达TrAP-INCENP F802A的INCENP沉默细胞（INCENP-OFF）中，内着丝粒蛋白（INCENP）的前中期定位以红色荧光标记。内源性生存素（Survivin）以绿色荧光标记，4',6-二脒基-2-苯基吲哚（DAPI，用于标记DNA）以蓝色荧光标记。相关细节可参见Xu等人2009年发表于《细胞生物学杂志（Journal of Cell Biology）》第187卷第637-653页的研究补充材料，即补充图2D的第1至4幅子图。