Bacterial composition of the subgingival biofilms after 3

The numerical composition of the individual bacterial species in the biofilms after 3 h and 24 h in co-culture with the multi-layered gingival epithelia was evaluated by quantitative real-time polymerase chain reaction (qPCR). The values represent bacterial numbers (mean ± SEM), from three independent biofilms in each group. Two-way ANOVA was used to calculate the differences between groups (P本研究采用实时荧光定量聚合酶链式反应（quantitative real-time polymerase chain reaction, qPCR），对多层牙龈上皮与生物膜共培养3小时及24小时后，生物膜中各细菌物种的数量组成进行了评估。各组数据均取自3个独立制备的生物膜，数值以细菌计数的平均值±标准误（standard error of the mean, SEM）表示。本研究采用双因素方差分析（Two-way ANOVA）计算各组间的差异（P