Aedes aegypti neurotranscriptome. Aedes aegypti strain:LVPIB12

To generate a complete neurotranscriptome of the Liverpool strain of Aedes aegypti, we performed Illumina RNA-sequencing (RNAseq) on 10 tissues from pools of female mosquitoes and 7 tissues from pools of male mosquitoes. These tissues include the brain, peripheral sensory tissues and organs (antennae, maxillary palp, proboscis, ovipositor/abdominal tip, legs, and ovary) and were replicated at least 3 times for each condition. Furthermore, to understand the influence of blood-feeding state on gene expression, we performed RNA-seq on a subset of tissues in female mosquitoes at three time-points: prior to a blood-meal (sugar-fed), at 48 hours following a blood-meal (blood-fed), and at 96 hours following a blood-meal (ovipositing). These experiments allowed us to characterize gene expression at high resolution in specific tissues, identify sexually-dimorphic gene expression, and to identify gene expression in female tissues that is regulated by blood-feeding state.

为构建埃及伊蚊（Aedes aegypti）利物浦株的完整神经转录组（neurotranscriptome），我们对雌性蚊子混池样本的10种组织与雄性蚊子混池样本的7种组织开展了Illumina RNA测序（Illumina RNA-sequencing, RNAseq）。本次测序涵盖的组织包括脑部、外周感觉组织与器官（触角、下颚须、喙、产卵器/腹部末端、足以及卵巢），且每种实验条件均至少重复3次。此外，为探究吸血状态对基因表达的调控作用，我们针对雌性蚊子的部分组织在三个时间点进行了RNA测序：吸血前（糖喂养状态）、吸血后48小时（吸血状态）以及吸血后96小时（产卵状态）。本实验系列实现了对特定组织中基因表达的高分辨率表征，鉴定了性别二态性基因表达模式，并识别出受吸血状态调控的雌性组织基因表达特征。