16S-V4 rRNA sequencing data from microcosm experiments using wetland sediment to investigate the influence of antibiotics and a nitrification inhibitor in agricultural run-off on N-cycling processes, 2019-2020

This project evaluated the discrete and combined effects of specific veterinary and human antibiotics and a common nitrification inhibitor (nitrapyrin) on nitrate-N treatment efficiency in wetland sediments. Sediment and water samples were collected from 3 locations at the USDA Meat Animal Research Facility near Clay Center NE in August 2019 for assessment of background conditions to determine the ideal collection site for future microcosm experiments to quantify rates of N-cycling activity in sediments. The sites ranged in historical exposure to inhibitors from (1) little to no relative historical exposure (Control site) (2) moderate relative historical exposure (Grade Control Structure 5), and (3) heavy relative historical exposure to antibiotic runoff from agricultural and cattle grazing land (Reuse Pit (RP)). Laboratory microcosm experiments were then initiated in December 2019 using sediment collected from the historically high exposure site (Reuse Pit) only. Microcosms were amended with either an antibiotic mixture (chlortetracycline, sulfadimethoxine, lincomycin, monensin) or nitrapyrin and the effect of the inhibitors on denitrification and nitrification rates and microbial community structure was quantified.

本项研究评估了特定兽医和人类抗生素以及一种常见的硝化抑制剂（硝吡菌）对湿地沉积物中硝酸盐-N处理效率的离散及联合影响。2019年8月，从美国农业部肉类动物研究设施（位于内布拉斯加州克莱中心东北部）的3个地点采集了沉积物和水质样本，以评估背景条件并确定未来微宇宙实验的理想采集地点，旨在量化沉积物中氮循环活动的速率。这些地点的历史暴露于抑制剂的程度有所不同，从（1）几乎没有相对历史暴露（对照组）到（2）中度相对历史暴露（等级控制结构5），再到（3）严重相对历史暴露，这是由于农业和牲畜放牧土地上的抗生素径流（复用坑（RP））。随后，于2019年12月启动了实验室微宇宙实验，仅使用从历史高暴露地点（复用坑）采集的沉积物。微宇宙被添加了抗生素混合物（氯四环素、磺胺二甲氧嘧啶、林可霉素、莫能菌素）或硝吡菌，并量化了抑制剂对反硝化和硝化速率以及微生物群落结构的影响。