Haemophilus influenzae strain C486 RNA SEQ NTHi modA4. Haemophilus influenzae strain C486 RNA SEQ NTHi modA4

comparison of RNA expression profiles from NTHi strain C486 strains expressing methyltransferase ModA4 (ON) or not (OFF) Overall design: RNA isolated from triplicate cultures of each strain (modA4 ON and modA4 OFF) grown in sBHI to mid-log; comparison of gene expression profiles by comparing modA4ON vs modA4 OFF to determine differential gene expression influenced by ModA2 methylation differences

表达甲基转移酶（methyltransferase）ModA4的NTHi菌株C486（开启组，ON）与不表达该酶的该菌株（关闭组，OFF）的RNA表达谱对比分析。实验设计概述：将modA4开启组与modA4关闭组的各三次生物学重复培养物，于补充型脑心浸液肉汤（sBHI）中培养至对数中期后提取RNA；通过对比modA4 ON与modA4 OFF的基因表达谱，以确定受ModA2甲基化差异影响的差异表达基因。