Gene expression data from remote ischemic postconditioning in rat model of ischemia reperfusion injury. Gene expression data from remote ischemic postconditioning in rat model of ischemia reperfusion injury

Restoration of blood flow is the definitive therapy to salvage myocardium following ischemic injury. However, sudden restoration of blood flow to the ischemic myocardium causes ischemia reperfusion injury (IRI). Here, the cardioprotective effect of remote ischemic postconditioning (RPostC) was investigated, based on our in vitro rat model of myocardial IRI. Three groups, including Sham, IRI, and IRI+ RPostC, were utilized for the analysis of Affymetrix Rat Gene 2.0 ST chip. Overall design: Total RNAs were extracted and purified from the heart tissues of three groups using TRIzol reagent (Life Technologies) and RNeasy mini kit (Qiagen). Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.). Affymetrix Human Transcriptome Array 2.0 was then utilized to hybridize the fragmented cRNA product. After washing and staining in the GeneChip Fluidics Station 450, Affymetrix® GeneChip Command Console (AGCC) and robust multichip analysis (RMA) algorithms were used to scan the arrays and analyze the data

缺血性损伤后恢复血流是挽救缺血心肌的确定性治疗手段，但缺血心肌的血流突然恢复会引发缺血再灌注损伤（ischemia reperfusion injury, IRI）。本研究基于体外大鼠心肌IRI模型，探究远程缺血后处理（remote ischemic postconditioning, RPostC）的心肌保护作用。实验设置假手术组（Sham）、IRI组、IRI+RPostC组三组，采用Affymetrix大鼠基因2.0 ST芯片（Affymetrix Rat Gene 2.0 ST chip）进行分析。整体实验设计如下：使用TRIzol试剂（Life Technologies公司）与RNeasy迷你试剂盒（Qiagen公司）从三组大鼠的心脏组织中提取并纯化总RNA；随后采用Ambion WT表达试剂盒（Affymetrix公司）合成cDNA，并通过GeneChip WT末端标记与对照试剂盒（Affymetrix, Inc.）制备cRNA；之后利用Affymetrix人类转录组芯片2.0（Affymetrix Human Transcriptome Array 2.0）对片段化cRNA产物进行杂交。在GeneChip Fluidics Station 450芯片工作站完成洗涤与染色后，采用Affymetrix® GeneChip Command Console（AGCC）软件及稳健多芯片分析（robust multichip analysis, RMA）算法对芯片进行扫描与数据分析。