Transcriptional response of Arabidopsis seedlings to light

We report the transcriptomic response of Arabidopsis thaliana seedlings grown in continuous white light, darkness or darkness and 1 hour of red light Overall design: Plants were germinated in true-dark conditions as described in (Leivar et al., 2008). Briefly, seeds were sterilized, plated in MS medium without sucrose, irradiated for 3h with white light to induce germination, followed by a saturating far-red light pulse during 5 min before being stratified for 4 days at 4ºC in darkness. They were grown in the dark at 21ºC for 2 days before being collected (D samples) or grown in the dark for 47 hours and treated with 10 µE·m-2·s-1 red light for 1 hour (R1 samples). White-light grown samples (WLc) were grown under continuous white-light (100 µE·m-2·s-1 PAR) after stratification for 2 days.

本研究报道了在连续白光、黑暗环境，或黑暗结合1小时红光处理下生长的拟南芥（Arabidopsis thaliana）幼苗的转录组响应。实验设计概述：实验材料参照Leivar等人2008年的研究方法，在完全黑暗条件下发芽。具体操作简述如下：种子经消毒后接种于不含蔗糖的MS培养基中，先用白光照射3小时诱导发芽，随后施加5分钟饱和远红光脉冲，再置于黑暗环境中4℃低温层积4天。部分幼苗于21℃黑暗环境中培养2天后取材（记为D组样本）；另一组幼苗先在黑暗中培养47小时，随后以10 μE·m⁻²·s⁻¹的红光处理1小时后取材（记为R1组样本）。白光培养样本（WLc）则在低温层积2天后，置于连续白光（100 μE·m⁻²·s⁻¹光合有效辐射（Photosynthetically Active Radiation，PAR））环境中培养。