The effects of differential gut microbiota on hepatic gene expression

Our preliminary data suggest that differential gut microbiota modulates acetaminophen-induced hepatotoxicity (APAP toxicity) in mice model. The goal of our study is to determine whether commensal gut microbiota modulates the hepatic gene expressions potentially responsible for modulating APAP toxicity. Method: C57BL/6 male mice from two different vendors [Jackson (JAX) and Taconic (TAC)] were sacrificed and liver tissue collected. Cecum materials from JAX or TAC mice were inoculated to germ-free (GF) mice. After 4 weeks of conventionalization, mice were overnight fasted (16 h) and sacrificed for liver collection. Examination of 5 groups; JAX, TAC, GF, GF_JAX and GF_TAC

我们的预实验数据表明，在小鼠模型中，差异肠道菌群可调控对乙酰氨基酚诱导的肝损伤（acetaminophen-induced hepatotoxicity，APAP毒性）。本研究的目标为探明共生肠道菌群是否可调控潜在参与APAP毒性调控的肝脏基因表达。方法：选取来自两家供应商[Jackson（JAX）与Taconic（TAC）]的C57BL/6雄性小鼠，将其处死并收集肝脏组织；将JAX或TAC小鼠的盲肠内容物移植至无菌（germ-free，GF）小鼠体内。经4周常规化定植后，对小鼠禁食过夜（16小时），随后处死并收集肝脏组织。实验共设置5组：JAX组、TAC组、GF组、GF_JAX组及GF_TAC组。