Raw calcium imaging data (2-1) for Generation of stable heading representations in diverse visual scenes

Two-photon imaging data for the following paper.Generation of stable heading representations in diverse visual scenesSung Soo Kim+, Ann M. Hermundstad, Sandro Romani, L. F. Abbott, Vivek Jayaraman+Nature (2019) 576:126-131Many animals rely on an internal heading representation when navigating in varied environments. How this representation is linked to the sensory cues that define different surroundings is unclear. In the fly brain, heading is represented by 'compass' neurons that innervate a ring-shaped structure known as the ellipsoid body. Each compass neuron receives inputs from 'ring' neurons that are selective for particular visual features; this combination provides an ideal substrate for the extraction of directional information from a visual scene. Here we combine two-photon calcium imaging and optogenetics in tethered flying flies with circuit modelling, and show how the correlated activity of compass and visual neurons drives plasticity, which flexibly transforms two-dimensional visual cues into a stable heading representation. We also describe how this plasticity enables the fly to convert a partial heading representation, established from orienting within part of a novel setting, into a complete heading representation. Our results provide mechanistic insight into the memory-related computations that are essential for flexible navigation in varied surroundings.Additional materials found in this Collection: https://doi.org/10.25378/janelia.c.4932540本数据集为下述论文配套的双光子成像（two-photon imaging）数据： 《多样视觉场景下稳定航向表征的构建》 作者：Sung Soo Kim+、Ann M. Hermundstad、Sandro Romani、L. F. Abbott、Vivek Jayaraman+ 发表于《自然》（Nature）2019年，第576卷：126-131页 许多动物在复杂环境中导航时，依赖于内部的航向表征。目前该表征与界定不同周遭环境的感官线索之间的关联机制仍不明确。在果蝇脑中，航向由一类被称为“罗盘神经元（compass neurons）”的细胞表征，这类神经元投射至被称为椭球体（ellipsoid body）的环状结构。每一枚罗盘神经元接收来自对特定视觉特征具有选择性的“环神经元（ring neurons）”的输入；这一连接为从视觉场景中提取方向信息提供了理想的神经底物。 本研究将双光子钙成像（two-photon calcium imaging）、光遗传学（optogenetics）技术应用于束缚式飞行果蝇，并结合环路建模（circuit modelling），揭示了罗盘神经元与视觉神经元的协同活性如何驱动可塑性（plasticity），进而将二维视觉线索灵活转化为稳定的航向表征。 本研究还阐释了该可塑性如何使果蝇能够将从全新环境局部定向过程中建立的局部航向表征，转换为完整的全局航向表征。本研究结果为灵活导航所需的记忆相关计算机制提供了机制层面的深入解析。 本数据集集合附带的补充材料可通过以下链接获取：https://doi.org/10.25378/janelia.c.4932540