Proteomic results from rice seed embryos subjected to controlled deterioration treatments (25°C, 40°C, 45°C; 83 RH ; 15 days)

Proteomic dataset on rice (Oryza sativa ssp japonica cv Nipponbare) seed embryos subjected to a controlled deterioration treatment at 25, 40 or 45°C during 15 days at 83% relative humidity (see below).

Protocol: control seeds refer to seeds kept at 4°C and 30% RH while other seed batches underwent controlled deterioration treatments. A high RH was used according to the described deterioration treatment (Tesnier et al. 2002). Seeds were equilibrated for 3 days at 83% RH at 25°C using a saturated solution of NaCl and control samples were immediately dried back for 3 days at 32% RH (25°C) in the presence of a saturated solution of CaCl2. The storage treatment at high RH was performed for various durations (from 1 to 15 d) by storing the seeds at 83% RH at either 25, 40 or 45°C. Seeds were then dried back for 3 days at 32% RH (25°C) and stored at 4°C in airtight tubes until germination tests.

本数据集为粳稻品种日本晴（Oryza sativa ssp. japonica cv. Nipponbare）种子胚的蛋白质组学数据，这些种子胚经相对湿度83%、温度25、40或45℃条件下的控制劣变处理，处理时长跨度为1至15天，详见下文。

实验方案：对照组种子为置于4℃、30%相对湿度（RH）条件下保存的种子，其余种子批次均接受控制劣变处理。本研究参照Tesnier等（2002）的方法，采用高湿度条件开展控制劣变处理。将种子置于饱和氯化钠溶液营造的环境中，在25℃、83% RH条件下平衡3天；对照组样品随即置于饱和氯化钙溶液营造的环境中，在32% RH、25℃条件下回干3天。将种子在83% RH条件下分别于25、40或45℃存储不同时长（1至15天）以完成处理。所有种子经处理后，均在32% RH、25℃条件下回干3天，随后装入密闭管中于4℃保存，直至开展发芽试验。