Impact of maturation and growth temperature on transcription profiles of Penicillium roqueforti conidia. Impact of maturation and growth temperature on transcription profiles of Penicillium roqueforti conidia

Purpose: The goal of this study is to compare differences between Penicillium roqueforti conidia from various conditions shown to affect heat-resistance to identify genes possibly related to heat-resistance using RNA-seq. Methods: mRNA from Penicillium roqueforti conidia produced at different temperatures (15C, 25C or 30C) and different cultivation times (3, 5, 7 or 10 days) was extracted, in triplicate, and sequenced with Illumina NextSeq500. The sequence reads that passed quality filters were analyzed using Hisat2 followed by Cufflinks. Results: we mapped about 19 million sequence reads per sample to the Penicillium roqueforti genome (build LCP96 04111) and identified 9723 transcripts in the conidia. Data analysis revealed a subset of 33 genes showing increased expression in culture conditions that increase heat-resistance. Conclusions: Our study generated insight into multiple genes that are likely involved in the heat-resistance of Penicillium roqueforti. Overall design: mRNA profiles of conidia from 3,5,7, and 10 day old Penicillium roqueforti cultures grown at 25C, and profiles of conidia from 7 day old cultures grown at 15C and 30C were generated, in triplicate, with deep sequencing using Illumina NextSeq 500.

研究目的：本研究旨在对比不同影响耐热性的培养条件下产生的娄地青霉（Penicillium roqueforti）分生孢子之间的差异，并通过RNA测序（RNA-seq）筛选可能与耐热性相关的基因。
实验方法：提取分别在15℃、25℃或30℃下培养3、5、7或10天所得的娄地青霉分生孢子的mRNA，设置三次生物学重复，采用Illumina NextSeq500平台完成测序。对通过质量过滤的测序读段，先使用Hisat2进行基因组比对，再通过Cufflinks进行转录组分析。
研究结果：每个样本约有1900万条测序读段可比对至娄地青霉基因组（组装版本LCP96 04111），并在分生孢子中鉴定出9723条转录本。数据分析显示，在可提升耐热性的培养条件下，共有33个基因的表达量显著上调。
研究结论：本研究为阐明参与娄地青霉耐热性调控的多个基因提供了关键研究视角。
整体实验设计：本研究生成两类转录组测序数据：其一为在25℃下分别培养3、5、7、10天的娄地青霉分生孢子的mRNA转录组；其二为分别在15℃、30℃下培养7天的娄地青霉分生孢子的mRNA转录组，所有样本均设置三次生物学重复，采用Illumina NextSeq 500平台进行深度测序。