Next Generation Sequencing Facilitates Qunatitative Analysis of MondoA+/+Foxp3Cre and MondoAfl/flFoxp3Cre Treg cells Transcriptomes

RNA-seq of Treg cells derived from MondoA+/+Foxp3Cre and MondoAfl/flFoxp3Cre mice. Overall design: Total RNA of nTregs, anti-CD3/CD28 antibody-stimulated nTregs and iTregs from MondoA+/+Foxp3Cre and MondoAfl/flFoxp3Cre mice were isolated and RNA-seq experiments were performed subsequently. mRNA profiles were further analyzed and compared according to standard protocols.

本数据集为来自MondoA+/+Foxp3Cre与MondoAfl/flFoxp3Cre小鼠的调节性T细胞（Treg）的RNA测序（RNA-seq）数据。总体实验设计：从上述两种基因型小鼠中分离天然调节性T细胞（nTregs）、抗CD3/CD28抗体刺激的天然调节性T细胞以及诱导性调节性T细胞（iTregs）的总RNA，随后开展RNA测序实验。后续按照标准实验流程对mRNA表达谱进行分析与对比。