Proemer2025-MuSK-CaMK2b-WesternBlot

This dataset contains all raw- and quantification data of Western Blots used in the associated manuscript. Data were collected on a Li-Cor Odyssey XF using either fluorescent or chemiluminescent detection techniques. Filenames are coded as follows:\ Blot#_Experiment_n#-method-antibody-(date) Abbreviations:\ HEK-OE = Overexpession of CaMK2b in heterologous cells\ CaMK2bKO = Knockout of CaMK2b in cultured muscle cells\ WCL = Whole cell lysates, Blots were probed with antibodies as indicated in the Manuscript\ IP = Immunoprecipitation of MuSK\ TissueIP-(date)-Mbnl1 = Immunoprecipitation of MuSK from muscle lysate of Mbnl1 mutant mice\ TissueIP-(date)-CaMK2bKO = Immunoprecipitation of MuSK from muscle lysate of CaMK2b mutant mice Antibody abbreviations are available from Supplementary Table S2. (2025-02-10)本数据集包含关联研究论文中使用的所有蛋白质免疫印迹(Western Blot)原始数据与定量数据。相关数据通过Li-Cor Odyssey XF成像系统采集，采用荧光检测与化学发光检测两种技术。 文件名编码规则如下：Blot编号_实验序号_n编号-检测方法-抗体-(日期) 缩写说明如下： HEK-OE：在异源细胞中过表达CaMK2b CaMK2bKO：在体外培养的肌细胞中敲除CaMK2b WCL：全细胞裂解液 印迹膜使用本论文中注明的抗体进行孵育检测 IP：对MuSK进行免疫沉淀 TissueIP-(日期)-Mbnl1：从Mbnl1基因突变小鼠的肌肉裂解液中免疫沉淀MuSK TissueIP-(日期)-CaMK2bKO：从CaMK2b基因突变小鼠的肌肉裂解液中免疫沉淀MuSK 抗体缩写详情请参见补充表S2。 (2025年2月10日)