SOX4 promotes T cell acute lymphoblastic leukemia progression

We report a previously unrecognized role of SOX4 in T cell acute lymphoblastic leukemia (T-ALL). To explore SOX4-dependent gene regulation, RNA-seq analysis was conducted and the differently expressed genes were revealed in the SOX4-knockdown Jurkat cells in comparison to control group. Overall design: mRNA profiles of Jurkat cells transfected with scramble shRNA (sh-Scb) or sh-SOX4 were generated by RNA sequencing, in double, using Illumina NovaSeq6000.

本研究报道了SOX4在T细胞急性淋巴细胞白血病（T-cell acute lymphoblastic leukemia, T-ALL）中一项此前未被发现的作用。为探究SOX4依赖的基因调控机制，本研究开展了RNA测序（RNA-seq）分析，对比SOX4敲低的Jurkat细胞与对照组细胞，成功鉴定得到差异表达基因（differently expressed genes）。实验整体设计：采用Illumina NovaSeq6000测序平台，对转染乱序短发夹RNA（scramble shRNA，sh-Scb）或靶向SOX4的短发夹RNA（sh-SOX4）的Jurkat细胞设置双生物学重复，通过RNA测序获取其mRNA表达谱（mRNA profiles）。