method for controlling sulfide and methane formation in sewer systems

Gene amplicon sequencing was conducted using a pair of universal prime (515f/806R) designed to target the variable regions V4-V5 of the microbial 16S RNA to explore the microbial diversity in sewer biofilms本研究采用一对靶向微生物16S RNA的V4-V5可变区的通用引物（515f/806R），开展基因扩增子测序（Gene amplicon sequencing），以探究污水生物膜中的微生物多样性。