Red blood cells TIF images

Raw data related to the paper entitled: Optical control of spatially localized red blood cell activity by holographic tweezingData type: Microscopy imagesImaging modality: High-speed brightfield microscopyCamera: PROMON U750 high-speed CMOS camera (AOS Technologies)Microscope: Custom-built microscopeObjective: Ob2 100x achromat oil-immersion objective with NA=1.45 (Nikon)Tube lens: 20 cm focal lengthSample: Red blood cells (RBCs) in phosphate-buffered saline (PBS) deposited on glassData description:The dataset consists of TIF images of RBCs captured using a high-speed CMOS camera operating at 1000 fps. The images were acquired using a custom-built microscope with a 100x oil-immersion objective and a 20 cm focal length tube lens. The RBCs were suspended in PBS and deposited on a glass coverslip.The dataset includes two types of images:Free-cell images: These images show RBCs freely flowing in PBS.Trapped-cell images: These images show RBCs trapped by holographic optical tweezers (HOT).The HOT are generated by a spatial light modulator (SLM) and focused onto the sample using a microscope objective. The SLM is programmed to create a pattern of light that traps the RBCs at specific locations.The dataset can be used to study the effects of optical trapping on RBCs. The data can also be used to develop and test new methods for optical manipulation of RBCs.Data organization:The dataset is organized into two compressed files .rar:Free_cells: This contains the free-cell images.Trapped_cells: This contains the trapped-cell images.Each folder contains a set of subfolder that specify the cel number. Each subfolder contains 5000 TIF files. The filenames are in the format captura_p0001.tif for example, for the first frame of the image sequence.本数据集关联的论文标题为：基于全息光镊的空间定位红细胞活性光学调控 数据类型：显微图像 成像模态：高速明场显微 相机：PROMON U750高速CMOS相机（AOS Technologies） 显微镜：定制搭建式显微镜 物镜：尼康Ob2 100倍油浸消色差物镜，数值孔径（Numerical Aperture, NA）=1.45 镜筒透镜：焦距20cm 样本：悬浮于磷酸盐缓冲液（Phosphate-Buffered Saline, PBS）中并沉积于玻片上的红细胞（Red Blood Cell, RBC） 数据说明：本数据集包含以1000帧每秒（frames per second, fps）帧率采集的红细胞TIF格式图像。图像采集系统采用定制搭建的显微镜，搭配100倍油浸物镜与20cm焦距镜筒透镜。实验所用红细胞悬浮于磷酸盐缓冲液中并沉积于玻璃盖玻片上。 本数据集包含两类图像： 1. 游离细胞图像：展示在磷酸盐缓冲液中自由流动的红细胞。 2. 捕获细胞图像：展示被全息光镊（Holographic Optical Tweezers, HOT）捕获的红细胞。 全息光镊由空间光调制器（Spatial Light Modulator, SLM）生成，并通过显微镜物镜聚焦至样本区域；空间光调制器经编程可生成特定光场图案，以在指定位置捕获红细胞。 本数据集可用于研究光捕获对红细胞的影响，也可用于开发与测试红细胞光学操控的新型方法。 数据组织：本数据集分为两个.rar压缩包： - Free_cells文件夹：内含游离细胞图像。 - Trapped_cells文件夹：内含捕获细胞图像。 每个文件夹下包含若干以细胞编号命名的子文件夹，每个子文件夹内包含5000张TIF格式图像，文件命名格式为captura_p0001.tif，例如对应图像序列的第一帧。