DataSheet_1_Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes.docx

Although first described over a hundred years ago, tissue optical clearing is undergoing renewed interest due to numerous advances in optical clearing methods, microscopy systems, and three-dimensional (3-D) image analysis programs. These advances are advantageous for intact mouse tissues or pieces of human tissues because samples sized several millimeters can be studied. Optical clearing methods are particularly useful for studies of the neuroanatomy of the central and peripheral nervous systems and tissue vasculature or lymphatic system. Using examples from solvent- and aqueous-based optical clearing methods, the mouse and human pancreatic structures and networks will be reviewed in 3-D for neuro-insular complexes, parasympathetic ganglia, and adipocyte infiltration as well as lymphatics in diabetes. Optical clearing with multiplex immunofluorescence microscopy provides new opportunities to examine the role of the nervous and circulatory systems in pancreatic and islet functions by defining their neurovascular anatomy in health and diabetes.

尽管组织光学清透（tissue optical clearing）技术于百余年前便已有相关描述，但近年来随着光学清透方法、显微成像系统及三维（3-D）图像分析程序的多项技术进展，该领域再度引发学界广泛关注。这些技术进展对于完整小鼠组织或人体组织切块而言极具应用价值，因其可支持对尺寸达数毫米的样本开展研究。光学清透方法尤其适用于中枢与外周神经系统的神经解剖学研究，以及组织脉管或淋巴系统的相关探究。本文将以基于溶剂与水相的光学清透方法为例，从三维视角综述小鼠与人体的胰腺结构及网络，涵盖糖尿病状态下的神经-胰岛复合体、副交感神经节、脂肪细胞浸润及淋巴系统相关情况。结合多重免疫荧光显微术（multiplex immunofluorescence microscopy）的光学清透技术，通过明确健康与糖尿病状态下的神经血管解剖结构，为探究神经系统与循环系统在胰腺及胰岛功能中的作用提供了全新契机。